Edna May Oliver portrays a society dowager called for jury duty on a murder trial wherein a pretty young woman is accused of killing her older husband. She takes her job quite seriously, and soon is playing both "prosecutor" and "DA" with judge and witnesses alike. In this unorthodox but highly entertaining fashion, Ms. Oliver gets to the truth and exposes the genuine murderer before the final fade-out. Incidentally, despite the title, there are gentlemen on the jury, but all eyes are on the formidable Ms. Oliver. Ladies of the Jury was remade in 1937 as We're on the Jury, with Helen Broderick in the Edna May Oliver role.